Nefm polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of Nefm on whole rat cerebellum homogenate using Nefm polyclonal antibody (Cat # PAB12103), at dilution of 1 : 20,000.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant Nefm.
Immunogen
Recombinant protein corresponding to C-terminus of rat Nefm.
Host
Rabbit
Theoretical MW (kDa)
145-170
Reactivity
Bovine, Chicken, Horse, Human, Mouse, Pig, Rat
Specificity
Specifically recognizes the evolutionarily conserved extreme C-terminal region of the medium neurofilament subunit (~145-170 KDa).
Form
Liquid
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
Immunofluorescence (1:500-1:000)
Western Blot (1:20000)
Immunocytochemistry (1:5000)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
The optimal working dilution should be determined by the end user.Storage Buffer
In antiserum
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Western blot analysis of Nefm on whole rat cerebellum homogenate using Nefm polyclonal antibody (Cat # PAB12103), at dilution of 1 : 20,000.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Frozen sections)
Immunocytochemistry
Immunofluorescence
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Gene Info — Nefm
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Publication Reference
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Preferential transformation of human neuronal cells by human adenoviruses and the origin of HEK 293 cells.
Shaw G, Morse S, Ararat M, Graham FL.
FASEB Journal 2002 Apr; 16(8):869.
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The bHLH gene hes1 as a repressor of the neuronal commitment of CNS stem cells.
Nakamura Y, Sakakibara S, Miyata T, Ogawa M, Shimazaki T, Weiss S, Kageyama R, Okano H.
Journal of Neuroscience 2000 Jan; 20(1):283.
Application：IHC-Fr, Mouse , Mouse brains.
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A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H.
Harris J, Ayyub C, Shaw G.
Journal of Neuroscience Research 1991 Sep; 30(1):47.
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Preferential transformation of human neuronal cells by human adenoviruses and the origin of HEK 293 cells.
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