SIAH1/SIAH2 monoclonal antibody, clone 8G7H12
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Recombinant protein)
SIAH1/SIAH2 monoclonal antibody, clone 8G7H12 (Cat # MAB2299) recognize both Drosophila SINA and human SIAH. There are two SINA-like E3 ligases, SINA and SINAH, in Drosophila.
SIAH1/SIAH2 monoclonal antibody, clone 8G7H12 is highly specific in recognizing both Drosophila SINA and human SIAH proteins but not Drosophila SINAH.
Coomassie blue staining patterns of both the wild-type (WT) and dominant negative (PD) forms of affinity-purified recombinant GST-SINA/SINAH/SIAH fusion proteins are shown in the top panel.
Protein standards were included and are shown in kDa.
Cross-reactivity of SIAH1/SIAH2 monoclonal antibody, clone 8G7H12 against Drosophila SINA and SINAH, and human hSIAH-1 and hSIAH-2 proteins were determined.
SIAH1/SIAH2 monoclonal antibody, clone 8G7H12 recognizes both human SIAH-1 and SIAH-2 protein.
Photo courtesy of Dr. Amy Tang, Mayo Clinic Cancer Center.
-
Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of SIAH1/SIAH2.
Immunogen
A synthetic peptide corresponding to amino acids 280-331 of drosophila SIAH1/SIAH2.
Host
Mouse
Reactivity
Fruit fly, Human, Pig, Rat, Zebra fish
Specificity
This antibody is specific to human SIAH 1 and 2 and Drosophila SINA in proliferating cells and stem cells. This antibody does not detect Drosophila SINAH or normal, nondividing cells.
Form
Liquid
Isotype
IgG1
Recommend Usage
Western Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In tissue culture supernatant (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Recombinant protein)
SIAH1/SIAH2 monoclonal antibody, clone 8G7H12 (Cat # MAB2299) recognize both Drosophila SINA and human SIAH. There are two SINA-like E3 ligases, SINA and SINAH, in Drosophila.
SIAH1/SIAH2 monoclonal antibody, clone 8G7H12 is highly specific in recognizing both Drosophila SINA and human SIAH proteins but not Drosophila SINAH.
Coomassie blue staining patterns of both the wild-type (WT) and dominant negative (PD) forms of affinity-purified recombinant GST-SINA/SINAH/SIAH fusion proteins are shown in the top panel.
Protein standards were included and are shown in kDa.
Cross-reactivity of SIAH1/SIAH2 monoclonal antibody, clone 8G7H12 against Drosophila SINA and SINAH, and human hSIAH-1 and hSIAH-2 proteins were determined.
SIAH1/SIAH2 monoclonal antibody, clone 8G7H12 recognizes both human SIAH-1 and SIAH-2 protein.
Photo courtesy of Dr. Amy Tang, Mayo Clinic Cancer Center.Immunoprecipitation
-
Publication Reference
-
Inhibition of RAS-mediated transformation and tumorigenesis by targeting the downstream E3 ubiquitin ligase seven in absentia homologue.
Schmidt RL, Park CH, Ahmed AU, Gundelach JH, Reed NR, Cheng S, Knudsen BE, Tang AH.
Cancer Research 2007 Dec; 67(24):11798.
Application：IHC-P, Human, Panc-1, MiaPaCa tumors from Mouse.
-
Inhibition of RAS-mediated transformation and tumorigenesis by targeting the downstream E3 ubiquitin ligase seven in absentia homologue.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com