PDIA3 monoclonal antibody, clone CL2444
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human PDIA3.
Immunogen
Recombinant protein corresponding to human PDIA3.
Epitope
This antibody binds to an epitope located within the peptide sequence KKFISDKDASIVGFF as determined by overlapping synthetic peptides.
Sequence
PTLKIFRDGEEAGAYDGPRTADGIVSHLKKQAGPASVPLRTEEEFKKFISDKDASIVGFFDDSFSEAHSEFLKAASNLRDNYRFAHTNVESLVNEYDDNGEGIILFRPSHLTNKFEDK
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Isotype
IgG1
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:5000-1:10000)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of U-251 MG cell lysate with PDIA3 monoclonal antibody, clone CL2444 (Cat # MAB15748).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human cerebral cortex with PDIA3 monoclonal antibody, clone CL2444 (Cat # MAB15748) shows strong cytoplasmic immunoreactivity in neurons.Immunofluorescence
Immunofluorescent staining of MCF7 cells with PDIA3 monoclonal antibody, clone CL2444 (Cat # MAB15748) (Green) shows specific endoplasmic reticulum. Microtubule and nuclear probes are visualized in red and blue respectively (where available). -
Gene Info — PDIA3
Entrez GeneID
2923Protein Accession#
P30101Gene Name
PDIA3
Gene Alias
ER60, ERp57, ERp60, ERp61, GRP57, GRP58, HsT17083, P58, PI-PLC
Gene Description
protein disulfide isomerase family A, member 3
Omim ID
602046Gene Ontology
HyperlinkGene Summary
This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. [provided by RefSeq
Other Designations
58 kDa microsomal protein|OTTHUMP00000041709|endoplasmic reticulum P58|glucose regulated protein, 58kDa|phospholipase C-alpha|protein disulfide isomerase-associated 3|protein disulfide-isomerase A3
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Interactome
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Pathway
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Disease
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