CD4 monoclonal antibody, clone CA-4
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Specification
Product Description
Mouse monoclonal antibody raised against CD4.
Immunogen
CD4-transfected mouse T-cell hybridoma, 3DT, followed by CD4+ human T-cell CEM cells.
Host
Mouse
Theoretical MW (kDa)
51
Reactivity
Human
Specificity
This antibody targets the intracellular domain of CD4.
Form
Lyophilized
Purification
Ascites
Isotype
IgG1
Recommend Usage
Flow Cytometry (1-3 ug/1x106 cells)
Immunocytochemistry (0.5-1 ug/ml)
Immunohistochemistry (Frozen sections) (1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from ascites fluid, 1.2% sodium acetate (2 mg BSA, 0.01 mg sodium azide).
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with 1 mL of PBS buffer and concentration will be 100 ug/mL, store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Frozen sections)
Immunocytochemistry
Flow Cytometry
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Gene Info — CD4
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Interactome
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Pathway
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Disease
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Publication Reference
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Immunohistochemical Evidence of the Involvement of Natural Killer (CD161+) Cells in Spontaneous Regression of Lewis Rat Sarcoma.
Kovalska J, Cervinkova M, Chmelikova E, Planska D, Cizkova J, Horak V.
In Vivo (Athens, Greece) 2019 Jan; 33(1):47.
Application:IF, IHC-Fr, Rat, Rat sarcoma.
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The immunological synapse: a molecular machine controlling T cell activation.
Grakoui A, Bromley SK, Sumen C, Davis MM, Shaw AS, Allen PM, Dustin ML.
Science 1999 Jul; 285(5425):221.
Application:Func, Mouse, Mouse T cells.
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Immunohistochemical Evidence of the Involvement of Natural Killer (CD161+) Cells in Spontaneous Regression of Lewis Rat Sarcoma.
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