ALDOA HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-ALDOA full length
Host
Human
Theoretical MW (kDa)
39
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ALDOA antibody (H00000226-M02) by Western Blots.
SDS-PAGE Gel
ALDOA transfected lysate
Western Blot
Lane 1: ALDOA transfected lysate ( 39 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
-
Applications
Western Blot
Immunoprecipitation
-
Gene Info — ALDOA
Entrez GeneID
226GeneBank Accession#
BC010660Protein Accession#
AAH10660Gene Name
ALDOA
Gene Alias
ALDA, MGC10942, MGC17716, MGC17767
Gene Description
aldolase A, fructose-bisphosphate
Omim ID
103850Gene Ontology
HyperlinkGene Summary
This gene product, Aldolase A (fructose-bisphosphate aldolase) is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants which encode the same protein. [provided by RefSeq
Other Designations
aldolase A|fructose-1,6-bisphosphate triosephosphate-lyase|fructose-bisphosphate aldolase A
-
Interactome
-
Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Carbon fixation in photosynthetic organisms
- Fructose and mannose metabolism
+ View More Disease
-
Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com