Glucose Uptake Assay Kit (Red Fluorescence)
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More Files
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Measurement of 2DG uptake in differentiated 3T3-L1 adipocytes and 3T3-L1 fibroblasts.
Measurement of 2DG uptake in differentiated 3T3-L1 adipocytes and 3T3-L1 fibroblasts.
Assays were performed with Glucose Uptake Assay Kit (Red Fluorescence) (Cat # KA4085) in a black wall/clear bottom cell culture Poly-D lysine plate using a Gemini (Molecular Devices) microplate reader. (A: Negative Control, no insulin no 2DG treatment. B: 2DG uptake in the absence of insulin. C: 2DG uptake in the presence of 1 uM insulin. D: 2DG uptake in the presence of 1 uM insulin and 200 uM phloretin. E: 2DG uptake in the presence of 1 uM insulin and 5mM D-Glucose.) (Please refer to the protocol for detailed operations).
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Specification
Product Description
Glucose Uptake Assay Kit (Red Fluorescence) is used for the measurement of glucose uptake.
Suitable Sample
Live Cells
Excitation/<br>Emission (nm)
540/590 nm
Detection Method
Fluorometric
Instrument Platform
Microplate Readers
Regulation Status
For research use only (RUO)
Storage Instruction
Store the kit in desiccated environment at -20°C and avoid from light.
Note
Measurement of 2DG uptake in differentiated 3T3-L1 adipocytes and 3T3-L1 fibroblasts.
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Applications
Quantification
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Publication Reference
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Silencing TCF4 Sensitizes Melanoma Cells to Vemurafenib Through Inhibiting GLUT3-Mediated Glycolysis.
Can Liu, Siqi He, Jianfei Zhang, Shiyan Li, Jian Chen, Chaofei Han.
OncoTargets and Therapy 2020 May; 13:4905.
Application：Func, Human, A375, SK-Mel-28 cells.
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Exercise Reduces Insulin Resistance in Type 2 Diabetes Mellitus via Mediating the lncRNA MALAT1/MicroRNA-382-3p/Resistin Axis.
Liu SX, Zheng F, Xie KL, Xie MR, Jiang LJ, Cai Y.
Molecular Therapy. Nucleic Acids 2019 Dec; 18:34.
Application：Func, Human, HUVECs.
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Silencing TCF4 Sensitizes Melanoma Cells to Vemurafenib Through Inhibiting GLUT3-Mediated Glycolysis.
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