KRT17 monoclonal antibody (M02), clone 2D10

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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant KRT17.
Immunogen
KRT17 (AAH00159, 1 a.a. ~ 432 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MTTSIRQFTSSSSIKGSSGLGGGSSRTSCRLSGGLGAGSCRLGSAGGLGSTLGGSSYSSCYSFGSGGGYGSSFGGVDGLLAGGEKATMQNLNDRLASYLDKVRALEEANTELEVKIRDWYQRQAPGPARDYSQYYRTIEELQNKILTATVDNANILLQIDNARLAADDFRTKFETEQALRLSVEADINGLRRVLDELTLARADLEMQIENLKEELAYLKKNHEEEMNALRGQVGGEINVEMDAAPGVDLSRIPNEMRDQYEKMAEKNRKDAEDWFFSKTEELNREVATNSELVQSGKSEISELRRTMQALEIELQSQLSMKASLEGNLAETENRYCVQLSQIQGLIGSVEEQLAQLRCEMEQQNQEYKILLDVKTRLEQEIATYRRLLEGEDAHLTQYKKEPVTTRQVRTIVEEVQDGKVISSREQVHQTTR
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (73.26 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of KRT17 expression in transfected 293T cell line by KRT17 monoclonal antibody (M02), clone 2D10.
Lane 1: KRT17 transfected lysate(48.1 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to KRT17 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged KRT17 is approximately 3ng/ml as a capture antibody.ELISA
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Gene Info — KRT17
Entrez GeneID
3872GeneBank Accession#
BC000159Protein Accession#
AAH00159Gene Name
KRT17
Gene Alias
K17, PC, PC2, PCHC1
Gene Description
keratin 17
Gene Ontology
HyperlinkGene Summary
This gene encodes the type I intermediate filament chain keratin 17, expressed in nail bed, hair follicle, sebaceous glands, and other epidermal appendages. Mutations in this gene lead to Jackson-Lawler type pachyonychia congenita and steatocystoma multiplex. [provided by RefSeq
Other Designations
cytokeratin-17
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Interactome
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Publication Reference
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Molecular classification of nonsmall cell lung cancer using a 4-protein quantitative assay.
Anagnostou VK, Dimou AT, Botsis T, Killiam EJ, Gustavson MD, Homer RJ, Boffa D, Zolota V, Dougenis D, Tanoue L, Gettinger SN, Detterbeck FC, Syrigos KN, Bepler G, Rimm DL.
Cancer 2012 Mar; 118(6):1607.
Application:IF, WB-Ti, Human, Nonsmall cell lung cancer.
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Molecular classification of nonsmall cell lung cancer using a 4-protein quantitative assay.
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