ARAF monoclonal antibody (M05), clone 3E2
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Specifications
Product Description
Mouse monoclonal antibody raised against a partial recombinant ARAF.
Immunogen
ARAF (NP_001645, 151 a.a. ~ 250 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
RQQFYHSVQDLSGGSRQHEAPSNRPLNELLTPQGPSPRTQHCDPEHFPFPAPANAPLQRIRSTSTPNVHMVSTTAPMDSNLIQLTGQSFSTDAAGSRGGS
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (83); Rat (82)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
ARAF monoclonal antibody (M05), clone 3E2 Western Blot analysis of ARAF expression in HeLa ( Cat # L013V1 ).Western Blot (Transfected lysate)
Western Blot analysis of ARAF expression in transfected 293T cell line by ARAF monoclonal antibody (M05), clone 3E2.
Lane 1: ARAF transfected lysate(67.6 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ARAF is approximately 0.1ng/ml as a capture antibody.ELISA
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between PKM2 and ARAF. HeLa cells were stained with anti-PKM2 rabbit purified polyclonal 1:1200 and anti-ARAF mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue). -
Gene Info — ARAF
Entrez GeneID
369GeneBank Accession#
NM_001654Protein Accession#
NP_001645Gene Name
ARAF
Gene Alias
A-RAF, ARAF1, PKS2, RAFA1
Gene Description
v-raf murine sarcoma 3611 viral oncogene homolog
Omim ID
311010Gene Ontology
HyperlinkOther Designations
OTTHUMP00000023212|Oncogene ARAF1|Ras-binding protein DA-Raf|v-raf murine sarcoma 3611 viral oncogene homolog 1
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Interactomes
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Pathways
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