MAPK8 monoclonal antibody (M01), clone 3H2
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Specifications
Product Description
Mouse monoclonal antibody raised against a partial recombinant MAPK8.
Immunogen
MAPK8 (NP_620637, 318 a.a. ~ 427 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
HPYINVWYDPSEAEAPPPKIPDKQLDEREHTIEEWKELIYKEVMDLEERTKNGVIRGQPSPLGAAVINGSQHPSSSSSVNDVSSMSTDPTLASDTDSSLEAAAGPLGCCR
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (38.21 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of MAPK8 expression in transfected 293T cell line by MAPK8 monoclonal antibody (M01), clone 3H2.
Lane 1: MAPK8 transfected lysate(48.3 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of MAPK8 transfected lysate using anti-MAPK8 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MAPK8 MaxPab rabbit polyclonal antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to MAPK8 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — MAPK8
Entrez GeneID
5599GeneBank Accession#
NM_139049Protein Accession#
NP_620637Gene Name
MAPK8
Gene Alias
JNK, JNK1, JNK1A2, JNK21B1/2, PRKM8, SAPK1
Gene Description
mitogen-activated protein kinase 8
Omim ID
601158Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq
Other Designations
JNK1 alpha protein kinase|JNK1 beta protein kinase|JUN N-terminal kinase|OTTHUMP00000019552|OTTHUMP00000019555|OTTHUMP00000019556|OTTHUMP00000019558|c-Jun N-terminal kinase 1|mitogen-activated protein kinase 8 isoform JNK1 alpha1|mitogen-activated protein
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Interactomes
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Diseases
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