ROCK2 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ROCK2.
Immunogen
A synthetic peptide corresponding to 15 amino acids near internal region of human ROCK2.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
Three isoforms of ROCK2 are known to exist; this antibody recognizes all three isoforms. ROCK2 antibody is predicted to not cross-react with other ROCK protein family members.
Form
Liquid
Purification
Peptide affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1-2 ug/mL)
Immunohistochemistry (5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of ROCK2 in mouse brain tissue lysate with ROCK2 polyclonal antibody (Cat # PAB19448) at 1 ug/mL (lane 1) and 2 ug/mL (lane 2).Immunohistochemistry
Immunohistochemical staining of mouse brain cells with ROCK2 polyclonal antibody (Cat # PAB19448) at 5 ug/mL.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ROCK2
Entrez GeneID
9475Protein Accession#
NP_004841Gene Name
ROCK2
Gene Alias
KIAA0619
Gene Description
Rho-associated, coiled-coil containing protein kinase 2
Omim ID
604002Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq
Other Designations
OTTHUMP00000195104
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Interactome
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