ROCK2 recombinant monoclonal antibody, clone 10E1
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human, mouse and rat ROCK2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to full length human ROCK2.
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunofluorescence(1:20-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: Hela whole cell lysate; Lane 2: MCF-7 whole cell lysate; Lane 3: L02 whole cell lysate; Lane 4: HepG2 whole cell lysate; Lane 5: Mouse Brain whole cell lysate; Lane 6: Rat Brain whole cell lysate.Immunofluorescence
Immunofluorescence staining of Hela Cells with ROCK2 recombinant monoclonal antibody, clone 10E1 at 1:50, counter-stained with DAPI.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ROCK2
Entrez GeneID
9475Protein Accession#
O75116Gene Name
ROCK2
Gene Alias
KIAA0619
Gene Description
Rho-associated, coiled-coil containing protein kinase 2
Omim ID
604002Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq
Other Designations
OTTHUMP00000195104
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Interactome
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