ROCK2 (phospho Y256) polyclonal antibody
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More Files
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Western Blot (Transfected lysate)
Western blot using ROCK2 (phospho Y256) polyclonal antibody (Cat # PAB11303) shows detection of phosphorylated ROCK2 in transfected 293T cells.
Lane 1 shows lysate expressing exogenous ROCK2 where the insert contains the transversion Y256A.
Lane 2 shows the lysate expressing WT ROCK2.
The primary antibody was used at a 1 : 1000 dilution.
Personal Communication, A. Papageorge and X. Qian, NCI, Bethesda, MD.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of ROCK2.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Y256 of human ROCK2.
Host
Rabbit
Reactivity
Human
Specificity
This antibody is specific to human ROCK-2 protein phosphorylated at Y256.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000-1:10000)
Western Blot (1:200-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot using ROCK2 (phospho Y256) polyclonal antibody (Cat # PAB11303) shows detection of phosphorylated ROCK2 in transfected 293T cells.
Lane 1 shows lysate expressing exogenous ROCK2 where the insert contains the transversion Y256A.
Lane 2 shows the lysate expressing WT ROCK2.
The primary antibody was used at a 1 : 1000 dilution.
Personal Communication, A. Papageorge and X. Qian, NCI, Bethesda, MD.Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
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Gene Info — ROCK2
Entrez GeneID
9475Protein Accession#
BAA75636; O75116Gene Name
ROCK2
Gene Alias
KIAA0619
Gene Description
Rho-associated, coiled-coil containing protein kinase 2
Omim ID
604002Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq
Other Designations
OTTHUMP00000195104
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Interactome
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Pathway
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Disease
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Publication Reference
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Rho GTPases: promising cellular targets for novel anticancer drugs.
Fritz G, Kaina B.
Current Cancer Drug Targets 2006 Feb; 6(1):1.
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Rho signalling at a glance.
Schwartz M.
Journal of Cell Science 2004 Nov; 117(Pt 23):5457.
Application：WB, Human, Cancers, Mammalian cells.
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Localization of the gene coding for ROCK II/Rho kinase on human chromosome 2p24.
Takahashi N, Tuiki H, Saya H, Kaibuchi K.
Genomics 1999 Jan; 55(2):235.
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Rho GTPases: promising cellular targets for novel anticancer drugs.
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