ROCK2 recombinant monoclonal antibody, clone R01-4F1
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More Files
- More Functions
Western Blot
Western blot analysis of ROCK2 in Hela, rat Brain, K562 lysates using human ROCK2 recombinant monoclonal antibody, clone R01-4F1 (Cat # RAB01725).
Immunocytochemistry
Immunocytochemical staining of Hela with ROCK2 recombinant monoclonal antibody, clone R01-4F1 (Cat # RAB01725). (A) ROCK2 (green) and (B) DAPI (blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against synthetic peptide of human ROCK2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human ROCK2
Theoretical MW (kDa)
Calculated MW: 161 k
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1:50-1:200)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of ROCK2 in Hela, rat Brain, K562 lysates using human ROCK2 recombinant monoclonal antibody, clone R01-4F1 (Cat # RAB01725).Immunocytochemistry
Immunocytochemical staining of Hela with ROCK2 recombinant monoclonal antibody, clone R01-4F1 (Cat # RAB01725). (A) ROCK2 (green) and (B) DAPI (blue). -
Gene Info — ROCK2
Entrez GeneID
9475Protein Accession#
O75116Gene Name
ROCK2
Gene Alias
KIAA0619
Gene Description
Rho-associated, coiled-coil containing protein kinase 2
Omim ID
604002Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq
Other Designations
OTTHUMP00000195104
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Interactome
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Pathway
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Disease
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