ROCK2 monoclonal antibody, clone ACDH-18
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic protein of human ROCK2.
Immunogen
A synthetic peptide corresponding to human ROCK2.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody reacts with human, mouse, rat ROCK2, in native form and recombinant. Superfamily members of ROCK2 are not reactive to antibody.
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1:50-200)
Immunocytochemistry (1:50-200)
Western Blot (1:1000-5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of (1) HeLa, (2) RAW264.7 cell lysates.Immunocytochemistry
Immunofluorescence
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Gene Info — ROCK2
Entrez GeneID
9475Protein Accession#
O75116Gene Name
ROCK2
Gene Alias
KIAA0619
Gene Description
Rho-associated, coiled-coil containing protein kinase 2
Omim ID
604002Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq
Other Designations
OTTHUMP00000195104
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Interactome
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