ISL1 monoclonal antibody, clone 1B1
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human ISL1.
Immunogen
Recombinant protein corresponding to human ISL1.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Recommend Usage
ELISA (1:10000)
Immunofluorescence (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascitic fluid (0.03% sodium azide).
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of using ISL1 monoclonal antibody, clone 1B1 against full-length Islet-1 (aa1-349)-hIgGFc transfected HEK293 cell lysate.Immunofluorescence
Immunofluorescence staining of HEK293 cells trasfected with full-length Islet-1-hIgGFc using ISL1 monoclonal antibody, clone 1B1 (Green). Blue: DRAQ5 fluorescent DNA dye.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ISL1
Entrez GeneID
3670Protein Accession#
P61371Gene Name
ISL1
Gene Alias
Isl-1
Gene Description
ISL LIM homeobox 1
Omim ID
600366Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young. [provided by RefSeq
Other Designations
ISL1 transcription factor, LIM/homeodomain|islet-1
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