ISL1 recombinant monoclonal antibody, clone 1A1
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of 293T whole cell lysate, K562 whole cell lysate, THP-1 whole cell lysate with ISL1 recombinant monoclonal antibody, clone 1A1 (Cat # RAB07414).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue using ISL1 recombinant monoclonal antibody, clone 1A1 (Cat # RAB07414) on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human ISL1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human ISL1.
Theoretical MW (kDa)
40
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography purification
Isotype
IgG
Recommend Usage
ELISA
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of 293T whole cell lysate, K562 whole cell lysate, THP-1 whole cell lysate with ISL1 recombinant monoclonal antibody, clone 1A1 (Cat # RAB07414).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue using ISL1 recombinant monoclonal antibody, clone 1A1 (Cat # RAB07414) on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ISL1
Entrez GeneID
3670Protein Accession#
P61371Gene Name
ISL1
Gene Alias
Isl-1
Gene Description
ISL LIM homeobox 1
Omim ID
600366Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young. [provided by RefSeq
Other Designations
ISL1 transcription factor, LIM/homeodomain|islet-1
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Interactome
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Disease
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