ISL1 monoclonal antibody, clone 1H9
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human ISL1.
Immunogen
Purified recombinant fragment corresponding to human ISL1 from E. coli.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Immunofluorescence (1:200-1:1000)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of HEK293 cell lysate with ISL1 monoclonal antibody, clone 1H9 (Cat # MAB16175).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human cortex with ISL1 monoclonal antibody, clone 1H9 (Cat # MAB16175).Immunofluorescence
Immunofluorescent staining of HEK293 cells with ISL1 monoclonal antibody, clone 1H9 (Cat # MAB16175) (Green) shows membrane localization.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ISL1
Entrez GeneID
3670Protein Accession#
P61371Gene Name
ISL1
Gene Alias
Isl-1
Gene Description
ISL LIM homeobox 1
Omim ID
600366Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young. [provided by RefSeq
Other Designations
ISL1 transcription factor, LIM/homeodomain|islet-1
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