ISL1 monoclonal antibody, clone 1H9
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis using ISL1 monoclonal antibody, clone 1H9 (Cat # MAB10702) against full-length ISL1 (aa 1-349) -hIgGFc transfected HEK293 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung cancer (upper) and cervical carcinoma (bottom) , showing nuclear localization using ISL1 monoclonal antibody, clone 1H9 (Cat # MAB10702) with DAB staining.
Immunofluorescence
Confocal immunofluorescence analysis of HEK293 cells trasfected with full-length ISL1-hIgGFc using ISL1 monoclonal antibody, clone 1H9 (Cat # MAB10702) (green) . Blue: DRAQ5 fluorescent DNA dye.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant ISL1.
Immunogen
Recombinant protein corresponding to human ISL1.
Host
Mouse
Theoretical MW (kDa)
39
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis using ISL1 monoclonal antibody, clone 1H9 (Cat # MAB10702) against full-length ISL1 (aa 1-349) -hIgGFc transfected HEK293 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung cancer (upper) and cervical carcinoma (bottom) , showing nuclear localization using ISL1 monoclonal antibody, clone 1H9 (Cat # MAB10702) with DAB staining.Immunofluorescence
Confocal immunofluorescence analysis of HEK293 cells trasfected with full-length ISL1-hIgGFc using ISL1 monoclonal antibody, clone 1H9 (Cat # MAB10702) (green) . Blue: DRAQ5 fluorescent DNA dye.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ISL1
Entrez GeneID
3670Gene Name
ISL1
Gene Alias
Isl-1
Gene Description
ISL LIM homeobox 1
Omim ID
600366Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young. [provided by RefSeq
Other Designations
ISL1 transcription factor, LIM/homeodomain|islet-1
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Interactome
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Disease
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