CDK2 recombinant monoclonal antibody, clone R04-1D5
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More Files
- More Functions
Western Blot
Western blot analysis of CDK2 in Jurkat, C6, CHO-K1, Hela lysates using CDK2 antibody.
Immunocytochemistry
Immunocytochemistry analysis of CDK2(green) in Hela using CDK2 antibody,and DAPI(blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human CDK2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to human Cdk2.
Theoretical MW (kDa)
Calculated MW: 34 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Frozen sections)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of CDK2 in Jurkat, C6, CHO-K1, Hela lysates using CDK2 antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Frozen sections)
Immunocytochemistry
Immunocytochemistry analysis of CDK2(green) in Hela using CDK2 antibody,and DAPI(blue).Immunofluorescence
Immunoprecipitation
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Gene Info — CDK2
Entrez GeneID
1017Protein Accession#
P24941Gene Name
CDK2
Gene Alias
p33(CDK2)
Gene Description
cyclin-dependent kinase 2
Omim ID
116953Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported. [provided by RefSeq
Other Designations
cdc2-related protein kinase|cell devision kinase 2|p33 protein kinase
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Interactome
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Pathway
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Disease
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