CDK2 (phospho Y15) recombinant monoclonal antibody, clone 2C4
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More Files
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Western Blot
Western blot analysis of Lane 1: Hela whole cell lysate (treated with Pervanadate), Lane 2: Hela whole cell lysate (not treated), Lane 3: 293 whole cell lysate (treated with Pervanadate) and Lane 4: 293 whole cell lysate (not treated) with CDK2 (phospho Y15) recombinant monoclonal antibody, clone 2C4 (Cat # RAB04259).
Immunocytochemistry
Immunocytochemical staining of HeLa cells with CDK2 (phospho Y15) recombinant monoclonal antibody, clone 2C4 (Cat # RAB04259) (diluated at 1:80).
Immunoprecipitation
Immunoprecipitation analysis of HeLa cell lysate with CDK2 (phospho Y15) recombinant monoclonal antibody, clone 2C4 (Cat # RAB04259).
Lane 1: rabbit control IgG, Lane 2: RAB04259 precipitates and Lane 3 : Input (Hela whole cell lysates).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human CDK2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding Y15 of human CDK2.
Theoretical MW (kDa)
Calculated MW: 34 kD
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunocytochemistry
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:200-1:1000)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: Hela whole cell lysate (treated with Pervanadate), Lane 2: Hela whole cell lysate (not treated), Lane 3: 293 whole cell lysate (treated with Pervanadate) and Lane 4: 293 whole cell lysate (not treated) with CDK2 (phospho Y15) recombinant monoclonal antibody, clone 2C4 (Cat # RAB04259).Immunohistochemistry
Immunocytochemistry
Immunocytochemical staining of HeLa cells with CDK2 (phospho Y15) recombinant monoclonal antibody, clone 2C4 (Cat # RAB04259) (diluated at 1:80).Immunoprecipitation
Immunoprecipitation analysis of HeLa cell lysate with CDK2 (phospho Y15) recombinant monoclonal antibody, clone 2C4 (Cat # RAB04259).
Lane 1: rabbit control IgG, Lane 2: RAB04259 precipitates and Lane 3 : Input (Hela whole cell lysates).Enzyme-linked Immunoabsorbent Assay
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Gene Info — CDK2
Entrez GeneID
1017Protein Accession#
P24941Gene Name
CDK2
Gene Alias
p33(CDK2)
Gene Description
cyclin-dependent kinase 2
Omim ID
116953Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported. [provided by RefSeq
Other Designations
cdc2-related protein kinase|cell devision kinase 2|p33 protein kinase
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