CDK1/CDK2 (phospho Thr14) recombinant monoclonal antibody, clone R06-1A5
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More Files
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Western Blot
Western blot analysis of Lane1: HepG2, Lane2: U2OS and Lane3: U251 lysates with CDK1/CDK2 (phospho Thr14) recombinant monoclonal antibody, clone R06-1A5 (Cat # RAB01793).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human CDK1/CDK2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding Thr14 of human CDK1/CDK2.
Theoretical MW (kDa)
Calculated MW: 34 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1/100)
Immunoprecipitation (1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane1: HepG2, Lane2: U2OS and Lane3: U251 lysates with CDK1/CDK2 (phospho Thr14) recombinant monoclonal antibody, clone R06-1A5 (Cat # RAB01793).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoprecipitation
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Gene Info — CDC2
Entrez GeneID
983Protein Accession#
P06493;P24941Gene Name
CDC2
Gene Alias
CDC28A, CDK1, DKFZp686L20222, MGC111195
Gene Description
cell division cycle 2, G1 to S and G2 to M
Omim ID
116940Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000019660|cell cycle controller CDC2|cell division control protein 2 homolog|cell division cycle 2 protein|cyclin-dependent kinase 1|p34 protein kinase
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Gene Info — CDK2
Entrez GeneID
1017Protein Accession#
P06493;P24941Gene Name
CDK2
Gene Alias
p33(CDK2)
Gene Description
cyclin-dependent kinase 2
Omim ID
116953Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported. [provided by RefSeq
Other Designations
cdc2-related protein kinase|cell devision kinase 2|p33 protein kinase
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Interactome
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Pathway
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Disease
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