NAMPT monoclonal antibody, clone 1D3A12
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More Files
- More Functions
Western Blot
Western blot analysis using NAMPT monoclonal antibody, clone 1D3A12 (Cat # MAB10403) against truncated NAMPT-His recombinant protein (1) and full-length GFP-NAMPT(aa1-491) transfected COS-7 cell lysate (2).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant NAMPT.
Immunogen
Recombinant protein corresponding to amino acids 338-479 of human NAMPT.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis using NAMPT monoclonal antibody, clone 1D3A12 (Cat # MAB10403) against truncated NAMPT-His recombinant protein (1) and full-length GFP-NAMPT(aa1-491) transfected COS-7 cell lysate (2).Enzyme-linked Immunoabsorbent Assay
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Gene Info — NAMPT
Entrez GeneID
10135Gene Name
NAMPT
Gene Alias
1110035O14Rik, DKFZp666B131, MGC117256, PBEF, PBEF1, VF, VISFATIN
Gene Description
nicotinamide phosphoribosyltransferase
Omim ID
608764Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein is an adipokine that is localized to the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It also activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity. This gene has a pseudogene on chromosome 10. [provided by RefSeq
Other Designations
NAmPRTase|pre-B cell-enhancing factor|pre-B-cell colony enhancing factor 1
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Interactome
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Pathway
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Disease
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