NAMPT monoclonal antibody, clone 4D5
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of 293T cell lysates.
Immunofluorescence
Immunofluorescence analysis of HeLa cells. The cell was stained with NAMPT monoclonal antibody, clone 4D5 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
-
Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant NAMPT.
Immunogen
Recombinant protein corresponding to full length human NAMPT.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG1, kappa
Recommend Usage
ELISA
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
Western blot analysis of 293T cell lysates.Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of HeLa cells. The cell was stained with NAMPT monoclonal antibody, clone 4D5 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
-
Gene Info — NAMPT
Entrez GeneID
10135GeneBank Accession#
NM_005746Protein Accession#
NP_005737Gene Name
NAMPT
Gene Alias
1110035O14Rik, DKFZp666B131, MGC117256, PBEF, PBEF1, VF, VISFATIN
Gene Description
nicotinamide phosphoribosyltransferase
Omim ID
608764Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein is an adipokine that is localized to the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It also activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity. This gene has a pseudogene on chromosome 10. [provided by RefSeq
Other Designations
NAmPRTase|pre-B cell-enhancing factor|pre-B-cell colony enhancing factor 1
-
Interactome
-
Pathway
-
Disease
-
Publication Reference
-
An update on visfatin/pre-B cell colony-enhancing factor, an ubiquitously expressed, illusive cytokine that is regulated in obesity.
Stephens JM, Vidal-Puig AJ.
Current Opinion in Lipidology 2006 Apr; 17(2):128.
-
Medicine. Visfatin: a new adipokine.
Hug C, Lodish HF.
Science 2005 Jan; 307(5708):366.
Application：ELISA, Human, Mouse, Adipose tissues.
-
An update on visfatin/pre-B cell colony-enhancing factor, an ubiquitously expressed, illusive cytokine that is regulated in obesity.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com