NAMPT monoclonal antibody, clone AF-1E12
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More Files
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Immunoprecipitation
IP-WB analysis of human NAMPT in cell lysates of three cell lines using this NAMPT monoclonal antibody, clone AF-1E12 (Cat # MAB8231) for IP and NAMPT polyclonal antibody (Cat # PAB17046) for WB.
Lane 1, 2 : Raji
Lane 3, 4 : HL-60
Lane 5, 6 : K-562
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant NAMPT.
Immunogen
Recombinant His fusion protein corresponding to full length human NAMPT.
Host
Mouse
Reactivity
Human
Specificity
This antibody detects endogenous NAMPT.
Form
Liquid
Isotype
IgG2a
Recommend Usage
Immunoprecipitation (1-2 ug/sample)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM phosphate buffer, 150 mM NaCl, pH 7.5 (50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunoprecipitation
IP-WB analysis of human NAMPT in cell lysates of three cell lines using this NAMPT monoclonal antibody, clone AF-1E12 (Cat # MAB8231) for IP and NAMPT polyclonal antibody (Cat # PAB17046) for WB.
Lane 1, 2 : Raji
Lane 3, 4 : HL-60
Lane 5, 6 : K-562Enzyme-linked Immunoabsorbent Assay
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Gene Info — NAMPT
Entrez GeneID
10135Gene Name
NAMPT
Gene Alias
1110035O14Rik, DKFZp666B131, MGC117256, PBEF, PBEF1, VF, VISFATIN
Gene Description
nicotinamide phosphoribosyltransferase
Omim ID
608764Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein is an adipokine that is localized to the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It also activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity. This gene has a pseudogene on chromosome 10. [provided by RefSeq
Other Designations
NAmPRTase|pre-B cell-enhancing factor|pre-B-cell colony enhancing factor 1
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Interactome
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Pathway
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Disease
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Publication Reference
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Circadian clock feedback cycle through NAMPT-mediated NAD+ biosynthesis.
Ramsey KM, Yoshino J, Brace CS, Abrassart D, Kobayashi Y, Marcheva B, Hong HK, Chong JL, Buhr ED, Lee C, Takahashi JS, Imai S, Bass J.
Science 2009 Mar; 324(5927):651.
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Circadian control of the NAD+ salvage pathway by CLOCK-SIRT1.
Nakahata Y, Sahar S, Astarita G, Kaluzova M, Sassone-Corsi P.
Science 2009 Mar; 324(5927):654.
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TNF regulates cellular NAD+ metabolism in primary macrophages.
Iqbal J, Zaidi M.
Biochemical and Biophysical Research Communications 2006 Apr; 342(4):1312.
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Circadian clock feedback cycle through NAMPT-mediated NAD+ biosynthesis.
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