NAMPT recombinant monoclonal antibody, clone R05-4C4
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More Files
- More Functions
Western Blot
Western blot analysis of Visfatin in 3T3, Raw264.7 lysates using Visfatin antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human lung cancer with NAMPT recombinant monoclonal antibody, clone R05-4C4 (Cat # RAB01241).
Immunocytochemistry
Western blot analysis of Visfatin in 3T3, Raw264.7 lysates using Visfatin antibody.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human NAMPT.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to human Visfatin.
Theoretical MW (kDa)
Calculated MW: 56 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Frozen sections)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Visfatin in 3T3, Raw264.7 lysates using Visfatin antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human lung cancer with NAMPT recombinant monoclonal antibody, clone R05-4C4 (Cat # RAB01241).Immunohistochemistry (Frozen sections)
Immunocytochemistry
Western blot analysis of Visfatin in 3T3, Raw264.7 lysates using Visfatin antibody.Immunofluorescence
Immunoprecipitation
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Gene Info — NAMPT
Entrez GeneID
10135Protein Accession#
P43490Gene Name
NAMPT
Gene Alias
1110035O14Rik, DKFZp666B131, MGC117256, PBEF, PBEF1, VF, VISFATIN
Gene Description
nicotinamide phosphoribosyltransferase
Omim ID
608764Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein is an adipokine that is localized to the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It also activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity. This gene has a pseudogene on chromosome 10. [provided by RefSeq
Other Designations
NAmPRTase|pre-B cell-enhancing factor|pre-B-cell colony enhancing factor 1
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Interactome
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Pathway
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Disease
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