VHH Bispecific Ab

The bispecific antibody (BsAb) is a type of recombinant antibody engineered to simultaneously bind to two different antigens or epitopes, facilitating various applications, such as redirecting T cells to tumor cells, dual targeting of disease mediators, blocking multiple signaling pathways, dual targeting of disease mediators, and delivering payloads to targeted sites.

Abnova offers custom services of four types of VHH bispecific antibodies: Bispecific VHH (No Fc) (nanoBiTE™), Bispecific VHH Mouse IgG, Bispecific VHH Rabbit IgG, and Bispecific VHH Human IgG. Traditional bispecific antibodies have inherent hydrophobic interactions in the VH and VL domains of engineered antibodies, limiting stability and solubility, and resulting in aggregation and mispairing of V domains. In contrast, VHH bispecific antibodies have a completely hydrophilic surface and do not bind light chains, allowing them to be easily linked into dimers by genetically fusing a second VHH antibody via a linker peptide to the N-terminus of a heavy-chain antibody. Such VHH-based bispecific antibodies show the advantages of small size, high solubility, high stability, and excellent tissue penetration in vivo.