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Pseudovirus Neutralizing Antibody Assay (Luciferase)

Abnova provides a fully integrated platform for high sensitivity and specificity quantification of COVID-19 neutralizing antibody. It consists of neutralizing antibody blockade of VSV-G lentivirus pseudotyped with COVID-19 spike (S) protein with luciferase (Luc) reporter gene interacting with ACE2 expressing 293T cells. Luc expression inside the ACE2 transfected stable 293T cells signifies viral entry and no neutralizing antibody blockade. In contrast, absence of Luc expression signifies the presence of neutralizing antibody. Luc reporter system enables a high sensitivity measurement of antibody neutralization. The platform’s specificity is determined by the pseudovirus S protein. Pseudovirus with different S proteins can constructed and generated for each COVID-19 variants (UK B1.1.7, South Africa B1.351) to differentiate neutralizing antibodies targeting specific variants.

See Technology
 
Flowchart

 
Spike Protein Selection and Pseudovirus Production

  • Spike Protein cDNA Selection (SARS-CoV-2, B1.1.7, B1.351)
  • Abnova’s Lentivirus Pseudotyped with S Protein (luciferase) and TCID50
  • Customer’s Pseudovirus (reporter gene) and TCID50
 
Target Cell Line Transfection

  • Abnova’s ACE2 Stable Transfected 293 T Cell Line
  • Customer’s ACE2 Transfected Cell Line
 
Neutralizing Antibody Assay Optimization and Testing

  • Pseduovirus and ACE2 Cell Line Assay Optimization
  • In-Process Assay QC (Abnova SARS-CoV-2 Neutralizing Antibody Cat #MAB22854-M07)
  • Plasma vs Serum Neutralizing Antibody Titers
  • IC50 Half-Maximum Inhibitory Concentration
 
Certification and Report

  • Biosafety Level 2 Facility
  • Neutralizing Antibody Report
 
Examples

 

 
  • COVID-19 SAM Vaccine: Abnova has developed a COVID-19 SAM RBD (receptor-binding domain) formulated in lipid nanoparticle (LNP) which generates higher antibody titer response than mRNA

 
 

For any inquiry, please contact : OEM@abnova.com

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