SEPT3 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
SEPT3 polyclonal antibody (Cat # PAB6153) (0.5 ug/mL) staining of Human Cerebellum (1), (0.1 ug/mL) Mouse (2) and (0.03 ug/mL) Rat (3) Brain lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
SEPT3 polyclonal antibody (Cat # PAB6153) (3.75 ug/mL) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of SEPT3.
Immunogen
A synthetic peptide corresponding to human SEPT3.
Sequence
SELVPEPRPKPA-C
Host
Goat
Theoretical MW (kDa)
40.7, 40.1
Reactivity
Human, Mouse, Rat
Specificity
This antibody is expected to recognize both reported isoforms according to NP_663786.1 and NP_061979.2.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Immunohistochemistry (3.75 ug/mL)
Western blot (0.03-0.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
SEPT3 polyclonal antibody (Cat # PAB6153) (0.5 ug/mL) staining of Human Cerebellum (1), (0.1 ug/mL) Mouse (2) and (0.03 ug/mL) Rat (3) Brain lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
SEPT3 polyclonal antibody (Cat # PAB6153) (3.75 ug/mL) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SEPT3
Entrez GeneID
55964Protein Accession#
NP_663786.1;NP_061979.2Gene Name
SEPT3
Gene Alias
MGC133218, SEP3, bK250D10.3
Gene Description
septin 3
Omim ID
608314Gene Ontology
HyperlinkGene Summary
This gene belongs to the septin family of GTPases. Members of this family are required for cytokinesis. Expression is upregulated by retinoic acid in a human teratocarcinoma cell line. The specific function of this gene has not been determined. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
OTTHUMP00000028742|neuronal-specific septin 3
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Interactome
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Disease
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Publication Reference
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Septin 3 (G-septin) is a developmentally regulated phosphoprotein enriched in presynaptic nerve terminals.
Xue J, Tsang CW, Gai WP, Malladi CS, Trimble WS, Rostas JA, Robinson PJ.
Journal of Neurochemistry 2004 Nov; 91(3):579.
Application：IF, WB-Ti, Rat, Brain, Intestine, Testis, Stomach, Kidney, Skeletal muscle, Pancreas, Lung, Liver, Heart, Spleen, Aorta, Adrenal gland.
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Septin 3 (G-septin) is a developmentally regulated phosphoprotein enriched in presynaptic nerve terminals.
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