C5AR1 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
C5AR1 polyclonal antibody (Cat # PAB27648) (0.01 ug/mL) staining of HepG2 cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
C5AR1 polyclonal antibody (Cat # PAB27648) (6 ug/mL) staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining. Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.
Immunofluorescence
C5AR1 polyclonal antibody (Cat # PAB27648) Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL).
Flow Cytometry
C5AR1 polyclonal antibody (Cat # PAB27648) Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
-
Specification
Product Description
Goat polyclonal antibody raised against peptide with C5AR1.
Immunogen
A synthetic peptide corresponding to C-RESKSFTRSTVDT, from the C Terminus of the protein sequence according to NP_001727.1.
Sequence
RESKSFTRSTVDT
Host
Goat
Reactivity
Human
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:64000)
Immunofluorescence (10 ug/mL)
Immunohistochemistry (6 - 8 ug/mL)
Flow Cytometry (10 ug/mL)
Western Blot (0.01 - 0.03 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
C5AR1 polyclonal antibody (Cat # PAB27648) (0.01 ug/mL) staining of HepG2 cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescenceImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
C5AR1 polyclonal antibody (Cat # PAB27648) (6 ug/mL) staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining. Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.Immunofluorescence
C5AR1 polyclonal antibody (Cat # PAB27648) Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL).Flow Cytometry
C5AR1 polyclonal antibody (Cat # PAB27648) Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody. -
Gene Info — C5AR1
Entrez GeneID
728Protein Accession#
NP_000048.1Gene Name
C5AR1
Gene Alias
C5A, C5AR, C5R1, CD88
Gene Description
complement component 5a receptor 1
Omim ID
113995Gene Ontology
HyperlinkOther Designations
C5a anaphylatoxin receptor|C5a ligand|complement component 5 receptor 1
-
Interactome
-
Pathway
-
Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com