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Last updated: 2023/3/19

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KEAP1 polyclonal antibody 

  • Catalog # : PAB19638
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of KEAP1.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 41-53 near N-terminus of human KEAP1.
  • Sequence:
  • C-EVTPSQHGNRTFS
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 69.7
  • Reactivity:
  • Human, Mouse
  • Specificity:
  • Reported variants represent identical protein: NP_036421.2, NP_987096.1
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Recommend Usage:
  • ELISA (1:32000)
    Immunofluorescence (5 ug/mL)
    Immunohistochemistry(3-5 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • KEAP1 polyclonal antibody (Cat # PAB19638) (3.8 ug/mL) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • Immunofluorescence
  • Immunofluorescence
  • KEAP1 polyclonal antibody (Cat # PAB19638) Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (5 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
  • Enzyme-linked Immunoabsorbent Assay
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Enzyme-linked Immunoabsorbent Assay
  • Gene Information
  • Entrez GeneID:
  • 9817
  • Gene Name:
  • KEAP1
  • Gene Alias:
  • INrf2,KIAA0132,KLHL19,MGC10630,MGC1114,MGC20887,MGC4407,MGC9454
  • Gene Description:
  • kelch-like ECH-associated protein 1
  • Gene Summary:
  • This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq
  • Other Designations:
  • cytosolic inhibitor of Nrf2
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