KEAP1 polyclonal antibody
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Specifications
Product Description
Goat polyclonal antibody raised against synthetic peptide of KEAP1.
Immunogen
A synthetic peptide corresponding to amino acids 41-53 near N-terminus of human KEAP1.
Sequence
C-EVTPSQHGNRTFS
Host
Goat
Theoretical MW (kDa)
69.7
Reactivity
Human, Mouse
Specificity
Reported variants represent identical protein: NP_036421.2, NP_987096.1
Form
Liquid
Purification
Antigen affinity purification
Recommend Usage
ELISA (1:32000)
Immunofluorescence (5 ug/mL)
Immunohistochemistry(3-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
KEAP1 polyclonal antibody (Cat # PAB19638) (3.8 ug/mL) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Immunofluorescence
KEAP1 polyclonal antibody (Cat # PAB19638) Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (5 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).Enzyme-linked Immunoabsorbent Assay
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Gene Info — KEAP1
Entrez GeneID
9817Protein Accession#
NP_036421.2Gene Name
KEAP1
Gene Alias
INrf2, KIAA0132, KLHL19, MGC10630, MGC1114, MGC20887, MGC4407, MGC9454
Gene Description
kelch-like ECH-associated protein 1
Omim ID
606016Gene Ontology
HyperlinkGene Summary
This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq
Other Designations
cytosolic inhibitor of Nrf2
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Interactomes
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Pathways
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Diseases
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