CDC42 polyclonal antibody
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More Files
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Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CDC42.
Immunogen
A synthetic peptide corresponding to internal region of human CDC42.
Host
Rabbit
Reactivity
Bovine, Human, Mouse, Rat
Form
Lyophilized
Purification
Immunoaffinity purification
Isotype
IgG
Recommend Usage
Western Blot (0.1-0.5 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.9 mg NaCl, 0.2 mg Na2HPO4 (5 mg BSA, 0.05 mg sodium azide, 0.05 mg Thimerosal)
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with 200 uL of deionized water and concentration will be 500 ug/mL, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide and thimerosal: POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of tissue and whole cell extracts with CDC42 polyclonal antibody (Cat # PAB19100).
Lane 1 : rat brain.
Lane 2 : MCF-7. Lane 3 : HeLa. Lane 4 : SMMC. Lane 5 : U87.Western Blot (Recombinant protein)
Western blot analysis of CDC42 recombinant protein with CDC42 polyclonal antibody (Cat # PAB19100).
Lane 1 : 10 ng.
Lane 2 : 5 ng.
Lane 3 : 2.5 ng.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
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Gene Info — CDC42
Entrez GeneID
998Gene Name
CDC42
Gene Alias
CDC42Hs, G25K
Gene Description
cell division cycle 42 (GTP binding protein, 25kDa)
Omim ID
116952Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq
Other Designations
GTP-binding protein, 25kD|OTTHUMP00000002834|OTTHUMP00000002926|cell division cycle 42|cell division cycle 42 (GTP binding protein, 25kD)|cell division cycle 42 (GTP-binding protein, 25kD)|dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD))|d
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Interactomes
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Pathways
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Diseases
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Publication Reference
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Eya1 protein phosphatase regulates tight junction formation in lung distal epithelium.
El-Hashash AH, Turcatel G, Varma S, Berika M, Al Alam D, Warburton D.
Journal of Cell Science 2012 Jun; 125(Pt 17):4036.
Application:WB, Mouse, MLE15 cells.
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Eya1 protein phosphatase regulates tight junction formation in lung distal epithelium.
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