RAN polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
RAN polyclonal antibody (Cat # PAB18990) (0.1 ug/mL) staining of Mouse (1) and Rat (2) Testis lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Western Blot (Tissue lysate)
RAN polyclonal antibody (Cat # PAB18990, 1 ug/mL) staining of human testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunofluorescence
RAN polyclonal antibody (Cat # PAB18990) Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of RAN.
Immunogen
A synthetic peptide corresponding to amino acids at C-terminus of human RAN.
Sequence
C-YEHDLEVAQTTALP
Host
Goat
Theoretical MW (kDa)
25
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:8000)
Immunofluorescence (10 ug/mL)
Western blot (0.1-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.5 mg/mL in Tris saline, pH7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
RAN polyclonal antibody (Cat # PAB18990) (0.1 ug/mL) staining of Mouse (1) and Rat (2) Testis lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.Western Blot (Tissue lysate)
RAN polyclonal antibody (Cat # PAB18990, 1 ug/mL) staining of human testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunofluorescence
RAN polyclonal antibody (Cat # PAB18990) Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).Enzyme-linked Immunoabsorbent Assay
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Gene Info — RAN
Entrez GeneID
5901Protein Accession#
NP_006316.1Gene Name
RAN
Gene Alias
ARA24, Gsp1, TC4
Gene Description
RAN, member RAS oncogene family
Omim ID
601179Gene Ontology
HyperlinkGene Summary
RAN (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The RAN protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of RAN requires the presence of regulator of chromosome condensation 1 (RCC1). Mutations in RAN disrupt DNA synthesis. Because of its many functions, it is likely that RAN interacts with several other proteins. RAN regulates formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. RAN could be a key signaling molecule regulating microtubule polymerization during mitosis. RCC1 generates a high local concentration of RAN-GTP around chromatin which, in turn, induces the local nucleation of microtubules. RAN is an androgen receptor (AR) coactivator that binds differentially with different lengths of polyglutamine within the androgen receptor. Polyglutamine repeat expansion in the AR is linked to Kennedy's disease (X-linked spinal and bulbar muscular atrophy). RAN coactivation of the AR diminishes with polyglutamine expansion within the AR, and this weak coactivation may lead to partial androgen insensitivity during the development of Kennedy's disease. [provided by RefSeq
Other Designations
OK/SW-cl.81|RanGTPase|guanosine triphosphatase Ran|member RAS oncogene family|ras-related nuclear protein
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Interactome
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Disease
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