CDKN1B (phospho T187) polyclonal antibody
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More Files
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Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of CDKN1B.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T187 of human CDKN1B.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
The CDKN1B (phospho T187) polyclonal antibody (Cat # PAB0560) is used in Western blot to detect Phospho-CDKN1B-T187 in HL-60 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with CDKN1B (phospho T187) polyclonal antibody (Cat # PAB0560) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma. -
Gene Info — CDKN1B
Entrez GeneID
1027Protein Accession#
NP_004055;P46527Gene Name
CDKN1B
Gene Alias
CDKN4, KIP1, MEN1B, MEN4, P27KIP1
Gene Description
cyclin-dependent kinase inhibitor 1B (p27, Kip1)
Gene Ontology
HyperlinkGene Summary
This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. [provided by RefSeq
Other Designations
cyclin-dependent kinase inhibitor 1B
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Interactomes
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Pathways
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Diseases
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Publication Reference
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Methylation status analysis of cell cycle regulatory genes (p16INK4A, p15INK4B, p21Waf1/Cip1, p27Kip1 and p73) in natural killer cell disorders.
Kawamata N, Inagaki N, Mizumura S, Sugimoto KJ, Sakajiri S, Ohyanagi-Hara M, Oshimi K.
European Journal of Haematology 2005 May; 74(5):424.
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BCR-ABL induces the expression of Skp2 through the PI3K pathway to promote p27Kip1 degradation and proliferation of chronic myelogenous leukemia cells.
Andreu EJ, Lledo E, Poch E, Ivorra C, Albero MP, Martinez-Climent JA, Montiel-Duarte C, Rifon J, Perez-Calvo J, Arbona C, Prosper F, Perez-Roger I.
Cancer Research 2005 Apr; 65(8):3264.
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The tumor-specific hyperactive forms of cyclin E are resistant to inhibition by p21 and p27.
Wingate H, Zhang N, McGarhen MJ, Bedrosian I, Harper JW, Keyomarsi K.
The Journal of Biological Chemistry 2005 Feb; 280(15):15148.
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Methylation status analysis of cell cycle regulatory genes (p16INK4A, p15INK4B, p21Waf1/Cip1, p27Kip1 and p73) in natural killer cell disorders.
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