BID (phospho S65) polyclonal antibody
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Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of BID.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S65 of human BID.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
BID (phospho S65) polyclonal antibody (Cat # PAB0419). Western blot analysis of BID expression in human colon.Western Blot (Cell lysate)
BID (phospho S65) polyclonal antibody (Cat # PAB0419). Western blot analysis of BID expression in Jurkat.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with BID (phospho S65) polyclonal antibody (Cat # PAB0419) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma. -
Gene Info — BID
Entrez GeneID
637Protein Accession#
NP_932070;P55957-2Gene Name
BID
Gene Alias
FP497, MGC15319, MGC42355
Gene Description
BH3 interacting domain death agonist
Omim ID
601997Gene Ontology
HyperlinkGene Summary
This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq
Other Designations
BH3-interacting domain death agonist|BID isoform ES(1b)|BID isoform L(2)|BID isoform Si6|Human BID coding sequence|OTTHUMP00000196197|apoptic death agonist|desmocollin type 4
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Interactomes
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Pathways
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Diseases
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Publication Reference
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The interaction between tBid and cardiolipin or monolysocardiolipin.
Liu J, Durrant D, Yang HS, He Y, Whitby FG, Myszka DG, Lee RM.
Biochemical and Biophysical Research Communications 2005 May; 330(3):865.
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Specific cleavage of Mcl-1 by caspase-3 in tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis in Jurkat leukemia T cells.
Weng C, Li Y, Xu D, Shi Y, Tang H.
The Journal of Biological Chemistry 2005 Mar; 280(11):10491.
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Bid mediates apoptotic synergy between tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and DNA damage.
Broaddus VC, Dansen TB, Abayasiriwardana KS, Wilson SM, Finch AJ, Swigart LB, Hunt AE, Evan GI.
The Journal of Biological Chemistry 2005 Apr; 280(13):12486.
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The interaction between tBid and cardiolipin or monolysocardiolipin.
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