MET (Y1230D) (Human) Recombinant Protein
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Specifications
Product Description
Human MET (NM_000245.2, 956 a.a. - 1390 a.a.) Y1230D mutant partial protein expressed in Sf9 cells.
Sequence
KKRKQIKDLGSELVRYDARVHTPHLDRLVSARSVSPTTEMVSNESVDYRATFPEDQFPNSSQNGSCRQVQYPLTDMSPILTSGDSDISSPLLQNTVHIDLSALNPELVQAVQHVVIGPSSLIVHFNEVIGRGHFGCVYHGTLLDNDGKKIHCAVKSLNRITDIGEVSQFLTEGIIMKDFSHPNVLSLLGICLRSEGSPLVVLPYMKHGDLRNFIRNETHNPTVKDLIGFGLQVAKGMKYLASKKFVHRDLAARNCMLDEKFTVKVADFGLARDMDDKEYYSVHNKTGAKLPVKWMALESLQTQKFTTKSDVWSFGVLLWELMTRGAPPYPDVNTFDITVYLLQGRRLLQPEYCPDPLYEVMLKCWHPKAEMRPSFSELVSRISAIFSTFIGEHYVHVNATYVNVKCVAPYPSLLSSEDNADDEVDTRPASFWETS
Host
insect
Theoretical MW (kDa)
78.739
Form
Liquid
Preparation Method
Insect cell (Sf9) expression system
Purification
GST affinity chromatography
Concentration
0.590 ug/uL
Activity
222 pmol/ug x min
Quality Control Testing
2 ug/lane SDS-PAGE Stained with Coomassie Blue
Storage Buffer
In 50 mM Hepes, 100 mM NaCl, pH 7.5 (5 mM DTT, 15 mM reduced glutathione, 20% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawingNote
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — MET
Entrez GeneID
4233Protein Accession#
NM_000245.2Gene Name
MET
Gene Alias
AUTS9, HGFR, RCCP2, c-Met
Gene Description
met proto-oncogene (hepatocyte growth factor receptor)
Gene Ontology
HyperlinkGene Summary
The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
HGF receptor|OTTHUMP00000069168|SF receptor|met proto-oncogene|met proto-oncogene tyrosine kinase|oncogene MET|scatter factor receptor
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Interactomes
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Pathways
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Diseases
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