Rabbit monoclonal antibody raised against synthetic protein of human OGT.
Immunogen:
A synthetic peptide corresponding to human OGT.
Host:
Rabbit
Reactivity:
Human, Mouse, Rat
Specificity:
This antibody reacts with human, mouse, rat OGT, in native form and recombinant. Superfamily members of OGT are not reactive to antibody.
Form:
Liquid
Purification:
Affinity purification
Isotype:
IgG
Recommend Usage:
Flow Cytometry (1:50) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-200) Immunofluorescence (1:50-200) Immunocytochemistry (1:50-200) Western Blot (1:500-2000) The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction:
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains nine tetratricopeptide repeats and a putative bipartite nuclear localization signal. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq