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UBE2I monoclonal antibody, clone EDF-21 

  • Catalog # : MAB20827
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic peptide of human UBE2I.
  • Immunogen:
  • A synthetic peptide corresponding to human UBE2I.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 18.007
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Flow Cytometry
    Immunocytochemistry (1:50-1:200)
    Immunofluorescence (1:50-1:200)
    Immunohistochemistry (1:50-1:200)
    Immunoprecipitation (1:50)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
  • Storage Instruction:
  • Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot analysis of HeLa cell lysate with UBE2I monoclonal antibody, clone EDF-21 (Cat # MAB20827).
  • Immunohistochemistry
  • Immunocytochemistry
  • Immunofluorescence
  • Immunoprecipitation
  • Flow Cytometry
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry
  • Immunocytochemistry
  • Immunofluorescence
  • Immunoprecipitation
  • Flow Cytometry
  • Gene Information
  • Entrez GeneID:
  • 7329
  • Gene Name:
  • UBE2I
  • Gene Alias:
  • C358B7.1,P18,UBC9
  • Gene Description:
  • ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
  • Gene Summary:
  • The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
  • Other Designations:
  • SUMO-1-protein ligase,ubiquitin carrier protein,ubiquitin conjugating enzyme 9,ubiquitin-conjugating enzyme E2I,ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9),ubiquitin-conjugating enzyme UbcE2A,ubiquitin-like protein SUMO-1 conjugating enzym
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