BOLL monoclonal antibody (M02), clone 1E3
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More Files
- More Functions
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Specifications
Product Description
Mouse monoclonal antibody raised against a partial recombinant BOLL.
Immunogen
BOLL (NP_149019, 185 a.a. ~ 283 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
ATTQYLPGQWQWSVPQPSASSAPFLYLQPSEVIYQPVEIAQDGGCVPPPLSLMETSVPEPYSDHGVQATYHQVYAPSAITMPAPVMQPEPIKTVWSIHY
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (92); Rat (92)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.63 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of BOLL expression in transfected 293T cell line by BOLL monoclonal antibody (M02), clone 1E3.
Lane 1: BOLL transfected lysate(31.301 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged BOLL is approximately 0.3ng/ml as a capture antibody.ELISA
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Gene Info — BOLL
Entrez GeneID
66037GeneBank Accession#
NM_033030Protein Accession#
NP_149019Gene Name
BOLL
Gene Alias
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Gene Description
bol, boule-like (Drosophila)
Omim ID
606165Gene Ontology
HyperlinkGene Summary
This gene belongs to the DAZ gene family required for germ cell development. It encodes an RNA-binding protein which is more similar to Drosophila Boule than to human proteins encoded by genes DAZ (deleted in azoospermia) or DAZL (deleted in azoospermia-like). Loss of this gene function results in the absence of sperm in semen (azoospermia). Histological studies demonstrated that the primary defect is at the meiotic G2/M transition. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq
Other Designations
boule
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Interactomes
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Diseases
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