TRIM32 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-TRIM32 full-length
Host
Human
Theoretical MW (kDa)
72
Interspecies Antigen Sequence
Mouse (96); Rat (96)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-TRIM32 antibody (H00022954-B01) by Western Blots.
SDS-PAGE Gel
TRIM32 transfected lysate.
Western Blot
Lane 1: TRIM32 transfected lysate ( 71.94 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — TRIM32
Entrez GeneID
22954GeneBank Accession#
BC003154.1Protein Accession#
AAH03154.1Gene Name
TRIM32
Gene Alias
BBS11, HT2A, LGMD2H, TATIP
Gene Description
tripartite motif-containing 32
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. The protein has also been localized to the nucleus, where it interacts with the activation domain of the HIV-1 Tat protein. The Tat protein activates transcription of HIV-1 genes. [provided by RefSeq
Other Designations
Limb-girdle muscular dystrophy 2H|OTTHUMP00000022763|TAT-interactive protein, 72-KD|limb girdle muscular dystrophy 2H (autosomal recessive)|muscular dystrophy, Hutterite type|tripartite motif protein TRIM32|zinc-finger protein HT2A
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Interactome
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Pathway
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Disease
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