WARS monoclonal antibody (M02), clone 3A12
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More Files
- More Functions
Western Blot (Cell lysate)
WARS monoclonal antibody (M02), clone 3A12 Western Blot analysis of WARS expression in HeLa ( Cat # L013V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of WARS expression in transfected 293T cell line by WARS monoclonal antibody (M02), clone 3A12.
Lane 1: WARS transfected lysate(53.2 KDa).
Lane 2: Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of WARS transfected lysate using anti-WARS monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with WARS MaxPab rabbit polyclonal antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to WARS on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (36.74 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant WARS.
Immunogen
WARS (NP_004175, 50 a.a. ~ 149 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
YKAAAGEDYKADCPPGNPAPTSNHGPDATEAEEDFVDPWTVQTSSAKGIDYDKLIVRFGSSKIDKELINRIERATGQRPHHFLRRGIFFSHRDMNQVLDA
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (84); Rat (84)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
WARS monoclonal antibody (M02), clone 3A12 Western Blot analysis of WARS expression in HeLa ( Cat # L013V1 ).Western Blot (Transfected lysate)
Western Blot analysis of WARS expression in transfected 293T cell line by WARS monoclonal antibody (M02), clone 3A12.
Lane 1: WARS transfected lysate(53.2 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of WARS transfected lysate using anti-WARS monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with WARS MaxPab rabbit polyclonal antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to WARS on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — WARS
Entrez GeneID
7453GeneBank Accession#
NM_004184Protein Accession#
NP_004175Gene Name
WARS
Gene Alias
GAMMA-2, IFI53, IFP53
Gene Description
tryptophanyl-tRNA synthetase
Omim ID
191050Gene Ontology
HyperlinkGene Summary
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. Tryptophanyl-tRNA synthetase (WARS) catalyzes the aminoacylation of tRNA(trp) with tryptophan and is induced by interferon. Tryptophanyl-tRNA synthetase belongs to the class I tRNA synthetase family. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq
Other Designations
interferon-induced protein 53|tryptophan tRNA ligase 1, cytoplasmic
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Interactome
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Pathway
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Disease
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