IRAK1 monoclonal antibody (M02), clone 3A9
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More Files
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Western Blot (Cell lysate)
IRAK1 monoclonal antibody (M02), clone 3A9 Western Blot analysis of IRAK1 expression in A-431 ( Cat # L015V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of IRAK1 expression in transfected 293T cell line by IRAK1 monoclonal antibody (M02), clone 3A9.
Lane 1: IRAK1 transfected lysate(68 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to IRAK1 on formalin-fixed paraffin-embedded human lung. [antibody concentration 3 ug/ml]
Immunoprecipitation
Immunoprecipitation of IRAK1 transfected lysate using anti-IRAK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with IRAK1 MaxPab rabbit polyclonal antibody.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged IRAK1 is approximately 0.03ng/ml as a capture antibody.
RNAi Knockdown (Antibody validated)
Western blot analysis of IRAK1 over-expressed 293 cell line, cotransfected with IRAK1 Validated Chimera RNAi ( Cat # H00003654-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with IRAK1 monoclonal antibody (M02) clone 3A9 (Cat # H00003654-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Immunofluorescence
Immunofluorescence of monoclonal antibody to IRAK1 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (43.45 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant IRAK1.
Immunogen
IRAK1 (AAH54000, 530 a.a. ~ 693 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
SSTGRAHSGAAPWQPLAAPSGASAQAAEQLQRGPNQPVESDESLGGLSAALRSWHLTPSCPLDPAPLREAGCPQGDTAGESSWGSGPGSRPTAVEGLALGSSASSSSEPPQIIINPARQKMVQKLALYEDGALDSLQLLSSSSLPGLGLEQDRQGPEESDEFQS
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (43.45 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
IRAK1 monoclonal antibody (M02), clone 3A9 Western Blot analysis of IRAK1 expression in A-431 ( Cat # L015V1 ).Western Blot (Transfected lysate)
Western Blot analysis of IRAK1 expression in transfected 293T cell line by IRAK1 monoclonal antibody (M02), clone 3A9.
Lane 1: IRAK1 transfected lysate(68 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to IRAK1 on formalin-fixed paraffin-embedded human lung. [antibody concentration 3 ug/ml]Immunoprecipitation
Immunoprecipitation of IRAK1 transfected lysate using anti-IRAK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with IRAK1 MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged IRAK1 is approximately 0.03ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of IRAK1 over-expressed 293 cell line, cotransfected with IRAK1 Validated Chimera RNAi ( Cat # H00003654-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with IRAK1 monoclonal antibody (M02) clone 3A9 (Cat # H00003654-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.Immunofluorescence
Immunofluorescence of monoclonal antibody to IRAK1 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — IRAK1
Entrez GeneID
3654GeneBank Accession#
BC054000Protein Accession#
AAH54000Gene Name
IRAK1
Gene Alias
IRAK, pelle
Gene Description
interleukin-1 receptor-associated kinase 1
Omim ID
300283Gene Ontology
HyperlinkGene Summary
This gene encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This gene is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000026014|OTTHUMP00000026020|Pelle homolog
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Interactome
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Pathway
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Disease
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