ALAS1 monoclonal antibody (M01), clone 3G10
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Specifications
Product Description
Mouse monoclonal antibody raised against a partial recombinant ALAS1.
Immunogen
ALAS1 (NP_000679, 1 a.a. ~ 98 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MESVVRRCPFLSRVPQAFLQKAGKSLLFYAQNCPKMMEVGAKPAPRALSTAAVHYQQIKETPPASEKDKTAKAKVQQTPDGSQQSPDGTQLPSGHPLP
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (86); Rat (83)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.52 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
ALAS1 monoclonal antibody (M01), clone 3G10 Western Blot analysis of ALAS1 expression in JAR ( Cat # L003V1 ).Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ALAS1 is approximately 0.1ng/ml as a capture antibody.ELISA
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Gene Info — ALAS1
Entrez GeneID
211GeneBank Accession#
NM_000688Protein Accession#
NP_000679Gene Name
ALAS1
Gene Alias
ALAS, ALAS3, ALASH, MIG4
Gene Description
aminolevulinate, delta-, synthase 1
Omim ID
125290Gene Ontology
HyperlinkGene Summary
Delta-aminolevulinate synthase (ALAS; EC 2.3.1.37) catalyzes the condensation of glycine with succinyl-CoA to form delta-aminolevulinic acid. This nuclear-encoded mitochondrial enzyme is the first and rate-limiting enzyme in the mammalian heme biosynthetic pathway. There are 2 tissue-specific isozymes: a housekeeping enzyme encoded by the ALAS1 gene and an erythroid tissue-specific enzyme encoded by ALAS2 (MIM 301300).[supplied by OMIM
Other Designations
aminolevulinate, delta, synthase 1|migration-inducing protein 4
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Interactomes
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Pathways
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Diseases
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Publication Reference
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PGC-1{alpha} is not mandatory for exercise- and training-induced adaptive gene responsens in mouse skeletal muscle.
Leick L, Wojtaszewski JF, Johansen ST, Kiilerich K, Comes G, Hellsten Y, Hidalgo J, Pilegaard H.
American Journal of Physiology. Endocrinology and Metabolism 2007 Dec; 294(2):E463.
Application:WB, Mouse, Mouse muscle.
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PGC-1{alpha} is not mandatory for exercise- and training-induced adaptive gene responsens in mouse skeletal muscle.
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