Western blots of neonatal rat brain (lane 1) and human recombinant SEMA3A chimera (95/125 kDa) (lane 2). Blots were probed with SEMA3A polyclonal antibody (Cat # PAB7888). The antibodies recognize both the 95 kDa and 125 kDa forms of the recombinant SEMA3A.
Formalin fixed, citric acid treated parafin sections of adult rat cerebellum. Sections were probed with SEMA3A polyclonal antibody (Cat # PAB7888) then anti-Rabbit HRP before detection using DAB. (Images provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).
This gene is a member of the semaphorin family and encodes a protein with an Ig-like C2-type (immunoglobulin-like) domain, a PSI domain and a Sema domain. This secreted protein can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Increased expression of this protein is associated with schizophrenia and is seen in a variety of human tumor cell lines. Also, aberrant release of this protein is associated with the progression of Alzheimer's disease. [provided by RefSeq