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Last updated: 2015/4/26
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SEMA3A polyclonal antibody

  • Catalog # : PAB7888
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of SEMA3A.
  • Immunogen:
  • A synthetic peptide corresponding to internal region of human SEMA3A.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • The sequence used is highly conserved in rat and mouse Sema3A, and has low homology to other semaphorin family members.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:2000)
    Western Blot (1:500)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot
  • Western Blot
  • Western blots of neonatal rat brain (lane 1) and human recombinant SEMA3A chimera (95/125 kDa) (lane 2). Blots were probed with SEMA3A polyclonal antibody (Cat # PAB7888). The antibodies recognize both the 95 kDa and 125 kDa forms of the recombinant SEMA3A.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin fixed, citric acid treated parafin sections of adult rat cerebellum. Sections were probed with SEMA3A polyclonal antibody (Cat # PAB7888) then anti-Rabbit HRP before detection using DAB. (Images provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).
  • ELISA
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Gene Name:
  • SEMA3A
  • Gene Alias:
  • Hsema-I,Hsema-III,MGC133243,SEMA1,SEMAD,SEMAIII,SEMAL,SemD,coll-1
  • Gene Description:
  • sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A
  • Gene Summary:
  • This gene is a member of the semaphorin family and encodes a protein with an Ig-like C2-type (immunoglobulin-like) domain, a PSI domain and a Sema domain. This secreted protein can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Increased expression of this protein is associated with schizophrenia and is seen in a variety of human tumor cell lines. Also, aberrant release of this protein is associated with the progression of Alzheimer's disease. [provided by RefSeq
  • Other Designations:
  • collapsin 1,semaphorin 3A,semaphorin D,semaphorin III,semaphorin L,semaphorin-like
  • Gene Pathway
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