PTPN1 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PTPN1.
Immunogen
A synthetic peptide corresponding to residues surrounding S50 of human PTPN1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to PTPN1.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:20000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from COS-7 cells, treated with UV (30 mins), using PTPN1 polyclonal antibody (Cat # PAB18287).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human brain tissue using PTPN1 polyclonal antibody (Cat # PAB18287).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
-
Gene Info — PTPN1
Entrez GeneID
5770Gene Name
PTPN1
Gene Alias
PTP1B
Gene Description
protein tyrosine phosphatase, non-receptor type 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation. [provided by RefSeq
Other Designations
OTTHUMP00000031266|non-receptor tyrosine phosphatase 1|protein tyrosine phosphatase 1B|protein tyrosine phosphatase, placental
-
Interactomes
-
Pathways
-
Diseases
-
Publication Reference
-
Protein-tyrosine phosphatase-1B (PTP1B) mediates the anti-migratory actions of Sprouty.
Yigzaw Y, Poppleton HM, Sreejayan N, Hassid A, Patel TB.
The Journal of Biological Chemistry 2003 Jan; 278(1):284.
Application:IP, WB-Tr, Human, HeLa cells.
-
PTP1B modulates the association of beta-catenin with N-cadherin through binding to an adjacent and partially overlapping target site.
Xu G, Arregui C, Lilien J, Balsamo J.
The Journal of Biological Chemistry 2002 Dec; 277(51):49989.
Application:ELISA, WB-Ce, WB-Tr, Chicken, Mouse, E8 neural retina, L cells.
-
Imaging sites of receptor dephosphorylation by PTP1B on the surface of the endoplasmic reticulum.
Haj FG, Verveer PJ, Squire A, Neel BG, Bastiaens PI.
Science 2002 Mar; 295(5560):1708.
Application:IF, Mouse, Mouse fibroblasts.
-
Protein-tyrosine phosphatase-1B (PTP1B) mediates the anti-migratory actions of Sprouty.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com