PTK2B polyclonal antibody
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More Files
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Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PTK2B.
Immunogen
A synthetic peptide corresponding to human PTK2B.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to PTK2B.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:20000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from NIH/3T3 cells, treated with Serum (20 %, 30 mins), using PTK2B polyclonal antibody (Cat # PAB18132).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human brain tissue using PTK2B polyclonal antibody (Cat # PAB18132).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — PTK2B
Entrez GeneID
2185Protein Accession#
Q14289Gene Name
PTK2B
Gene Alias
CADTK, CAKB, FADK2, FAK2, FRNK, PKB, PTK, PYK2, RAFTK
Gene Description
PTK2B protein tyrosine kinase 2 beta
Omim ID
601212Gene Ontology
HyperlinkGene Summary
This gene encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq
Other Designations
CAK beta|OTTHUMP00000128275|OTTHUMP00000162913|calcium-dependent tyrosine kinase|cell adhesion kinase beta|focal adhesion kinase 2|proline-rich tyrosine kinase 2|protein kinase B|protein tyrosine kinase 2 beta|related adhesion focal tyrosine kinase
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Interactomes
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Pathways
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Diseases
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Publication Reference
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PYK2 mediates anti-apoptotic AKT signaling in response to benzo[a]pyrene diol epoxide in mammary epithelial cells.
Burdick AD, Ivnitski-Steele ID, Lauer FT, Burchiel SW.
Carcinogenesis 2006 Nov; 27(11):2331.
Application:WB-Ce, Human, MCF10A cells.
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Implication of phospholipase D2 in oxidant-induced phosphoinositide 3-kinase signaling via Pyk2 activation in PC12 cells.
Banno Y, Ohguchi K, Matsumoto N, Koda M, Ueda M, Hara A, Dikic I, Nozawa Y.
The Journal of Biological Chemistry 2005 Apr; 280(16):16319.
Application:IP, WB, Rat, PC-12 cells.
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Cardiomyocyte apoptosis triggered by RAFTK/pyk2 via Src kinase is antagonized by paxillin.
Melendez J, Turner C, Avraham H, Steinberg SF, Schaefer E, Sussman MA.
Journal of Biological Chemistry 2004 Dec; 279(51):53516.
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PYK2 mediates anti-apoptotic AKT signaling in response to benzo[a]pyrene diol epoxide in mammary epithelial cells.
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