APEX1 polyclonal antibody
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Specifications
Product Description
Rabbit polyclonal antibody raised against recombinant APEX1.
Immunogen
Recombinant protein corresponding to human APEX1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to the human APE/ref-1protein.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunoprecipitation (7 ug/mL)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris-glycine, 150 mM NaCl (0.05% sodium azide)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of APEX1 in HeLa and 3T3 cell lysates using APEX1 polyclonal antibody (Cat # PAB12487).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Frozen sections)
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
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Gene Info — APEX1
Entrez GeneID
328Protein Accession#
P27695Gene Name
APEX1
Gene Alias
APE, APE-1, APE1, APEN, APEX, APX, HAP1, REF-1, REF1
Gene Description
APEX nuclease (multifunctional DNA repair enzyme) 1
Omim ID
107748Gene Ontology
HyperlinkGene Summary
Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein. [provided by RefSeq
Other Designations
AP endonuclease class I|AP lyase|APEX nuclease|DNA-(apurinic or apyrimidinic site) lyase|OTTHUMP00000163988|apurinic/apyrimidinic (abasic) endonuclease|apurinic/apyrimidinic exonuclease|deoxyribonuclease (apurinic or apyrimidinic)|multifunctional DNA repa
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Interactomes
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Pathways
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Diseases
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Publication Reference
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Effect of hypoxia on the expression of phosphoglycerate kinase and antitumor activity of troxacitabine and gemcitabine in non-small cell lung carcinoma.
Lam W, Bussom S, Cheng YC.
Molecular Cancer Therapeutics 2009 Feb; 8(2):415.
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Down-regulation of apurinic/apyrimidinic endonuclease 1/redox factor-1 expression by soy isoflavones enhances prostate cancer radiotherapy in vitro and in vivo.
Raffoul JJ, Banerjee S, Singh-Gupta V, Knoll ZE, Fite A, Zhang H, Abrams J, Sarkar FH, Hillman GG.
Cancer Research 2007 Mar; 67(5):2141.
Application:IF, IHC, WB, Human, Mouse, Mouse prostate tumors, PC-3 cells.
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Virion-associated uracil DNA glycosylase-2 and apurinic/apyrimidinic endonuclease are involved in the degradation of APOBEC3G-edited nascent HIV-1 DNA.
Yang B, Chen K, Zhang C, Huang S, Zhang H.
The Journal of Biological Chemistry 2007 Apr; 282(16):11667.
Application:WB-Tr, Human, HEK 293T cells.
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Human monocytes, but not dendritic cells derived from them, are defective in base excision repair and hypersensitive to methylating agents.
Briegert M, Kaina B.
Cancer Research 2007 Jan; 67(1):26.
Application:Func, WB-Ce, Human, Human monocytes, monocyte-derived dendritic cells.
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Expansion of myeloid immune suppressor Gr+CD11b+ cells in tumor-bearing host directly promotes tumor angiogenesis.
Yang L, DeBusk LM, Fukuda K, Fingleton B, Green-Jarvis B, Shyr Y, Matrisian LM, Carbone DP, Lin PC.
Cancer Cell 2004 Oct; 6(4):409.
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Effect of hypoxia on the expression of phosphoglycerate kinase and antitumor activity of troxacitabine and gemcitabine in non-small cell lung carcinoma.
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