Western blot (non - reducing) on Jurkat cell lysate using rabbit ATG16L1 polyclonal antibody (Cat # PAB0739) .
Immunohistochemistry (Frozen sections)
Confocal microscopy on immunofluorescently detected cytospinned HL-60 cell line. HL-60 cells were fixed with acetone 90 seconds ; primary antibody was diluted 1 : 100 and incubated for 1 hour, 3 x washes and secondary (Goat anti Rb 1 : 100 alexa 546 conjugate) for 30 minutes. Eosinophils seem to be stained using rabbit ATG16L1 polyclonal antibody (Cat # PAB0739) .
Autophagy is the major intracellular degradation system delivering cytoplasmic components to lysosomes, and it accounts for degradation of most long-lived proteins and some organelles. Cytoplasmic constituents, including organelles, are sequestered into double-membraned autophagosomes, which subsequently fuse with lysosomes. ATG16L1 is a component of a large protein complex essential for autophagy (Mizushima et al., 2003 [PubMed 12665549]).[supplied by OMIM
APG16 autophagy 16-like,APG16L beta,ATG16 autophagy related 16-like protein 1,WD repeat domain 30