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Last updated: 2014/4/14
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BIOtech
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ATG16L1 polyclonal antibody

  • Catalog # : PAB0739
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of ATG16L1.
  • Immunogen:
  • A synthetic peptide (conjugated with Blue Carrier Protein) corresponding to N-terminus of human ATG16L1.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Rat
  • Form:
  • Lyophilized
  • Recommend Usage:
  • Immunohistochemistry (1:100-1:3000)
    Immunofluorescence (1:100-1:3000)
    Western Blot (1:100-1:3000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from PBS
  • Storage Instruction:
  • Store at 4°C on dry atmosphere.
    After reconstitution with deionized water, store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot (non - reducing) on Jurkat cell lysate using rabbit ATG16L1 polyclonal antibody (Cat # PAB0739) .
  • Immunohistochemistry (Frozen sections)
  • Immunofluorescence
  • Immunofluorescence
  • Confocal microscopy on immunofluorescently detected cytospinned HL-60 cell line. HL-60 cells were fixed with acetone 90 seconds ; primary antibody was diluted 1 : 100 and incubated for 1 hour, 3 x washes and secondary (Goat anti Rb 1 : 100 alexa 546 conjugate) for 30 minutes. Eosinophils seem to be stained using rabbit ATG16L1 polyclonal antibody (Cat # PAB0739) .
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Frozen sections)
  • Gene Information
  • Gene Name:
  • ATG16L1
  • Gene Alias:
  • APG16L,ATG16L,FLJ00045,FLJ10035,FLJ10828,FLJ22677,IBD10,WDR30
  • Gene Description:
  • ATG16 autophagy related 16-like 1 (S. cerevisiae)
  • Gene Summary:
  • Autophagy is the major intracellular degradation system delivering cytoplasmic components to lysosomes, and it accounts for degradation of most long-lived proteins and some organelles. Cytoplasmic constituents, including organelles, are sequestered into double-membraned autophagosomes, which subsequently fuse with lysosomes. ATG16L1 is a component of a large protein complex essential for autophagy (Mizushima et al., 2003 [PubMed 12665549]).[supplied by OMIM
  • Other Designations:
  • APG16 autophagy 16-like,APG16L beta,ATG16 autophagy related 16-like protein 1,WD repeat domain 30
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