TP53 (phospho S20) polyclonal antibody
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Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of TP53.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S20 of human TP53.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of TP53 (phospho S20) polyclonal antibody (Cat # PAB0561) in A2058 cell lysate. TP53 (arrow) was detected using the purified polyclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with TP53 (phospho S20) polyclonal antibody (Cat # PAB0561) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma. -
Gene Info — TP53
Entrez GeneID
7157Protein Accession#
NP_000537;P04637Gene Name
TP53
Gene Alias
FLJ92943, LFS1, TRP53, p53
Gene Description
tumor protein p53
Gene Ontology
HyperlinkGene Summary
This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms, which can regulate p53 transcriptional activity. [provided by RefSeq
Other Designations
p53 antigen|p53 transformation suppressor|p53 tumor suppressor|phosphoprotein p53|transformation-related protein 53
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Interactomes
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Pathways
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Diseases
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Publication Reference
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Guilt by association? p53 and the development of aneuploidy in cancer.
Duensing A, Duensing S.
Biochemical and Biophysical Research Communications 2005 Jun; 331(3):694.
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p53-A pro-apoptotic signal transducer involved in AIDS.
Castedo M, Perfettini JL, Piacentini M, Kroemer G.
Biochemical and Biophysical Research Communications 2005 Jun; 331(3):701.
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The multiple roles of p53 in the pathogenesis of HIV associated dementia.
Garden GA, Morrison RS.
Biochemical and Biophysical Research Communications 2005 Jun; 331(3):799.
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Guilt by association? p53 and the development of aneuploidy in cancer.
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