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Last updated: 2015/8/23
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BLNK monoclonal antibody, clone 5G9

  • Catalog # : MAB10505
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant BLNK.
  • Immunogen:
  • Recombinant protein corresponding to human BLNK.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 68
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Immunofluorescence (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis using BLNK monoclonal antibody, clone 5G9 (Cat # MAB10505) against NIH/3T3 (1) and BCBL-1 (2) cell lysate.
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot analysis using BLNK monoclonal antibody, clone 5G9 (Cat # MAB10505) against human BLNK (aa: 34-216) recombinant protein. (Expected MW is 60 kDa).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using BLNK monoclonal antibody, clone 5G9 (Cat # MAB10505) with DAB staining.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of HepG2 cells using BLNK monoclonal antibody, clone 5G9 (Cat # MAB10505) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • ELISA
  • ELISA
  • ELISA detection with BLNK monoclonal antibody, clone 5G9 (Cat # MAB10505).
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of NIH/3T3 cells using BLNK monoclonal antibody, clone 5G9 (Cat # MAB10505) (green) and negative control (purple).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Gene Name:
  • BLNK
  • Gene Alias:
  • BASH,BLNK-S,LY57,MGC111051,SLP-65,SLP65
  • Gene Description:
  • B-cell linker
  • Gene Summary:
  • This gene encodes a cytoplasmic linker or adaptor protein that plays a critical role in B cell development. This protein bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. The phosphorylation of five tyrosine residues is necessary for this protein to nucleate distinct signaling effectors following B cell receptor activation. Mutations in this gene cause hypoglobulinemia and absent B cells, a disease in which the pro- to pre-B-cell transition is developmentally blocked. Deficiency in this protein has also been shown in some cases of pre-B acute lymphoblastic leukemia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • B cell linker protein,B-cell adapter containing a SH2 domain protein,B-cell adapter containing a Src homology 2 domain protein,OTTHUMP00000020167,Src homology 2 domain-containing leukocyte protein of 65 kDa
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