RALBP1 monoclonal antibody (M02), clone 2A1
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specifications
Product Description
Mouse monoclonal antibody raised against a full length recombinant RALBP1.
Immunogen
RALBP1 (AAH13126.1, 1 a.a. ~ 655 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MTECFLPPTSSPSEHRRVEHGSGLTRTPSSEEISPTKFPGLYRTGEPSPPHDILHEPPDVVSDDEKDHGKKKGKFKKKEKRTEGYAAFQEDSSGDEAESPSKMKRSKGIHVFKKPSFSKKKEKDFKIKEKPKEEKHKEEKHKEEKHKEKKSKDLTAADVVKQWKEKKKKKKPIQEPEVPQIDVPNLKPIFGIPLADAVERTMMYDGIRLPAVFRECIDYVEKYGMKCEGIYRVSGIKSKVDELKAAYDREESTNLEDYEPNTVASLLKQYLRDLPENLLTKELMPRFEEACGRTTETEKVQEFQRLLKELPECNYLLISWLIVHMDHVIAKELETKMNIQNISIVLSPTVQISNRVLYVFFTHVQELFGNVVLKQVMKPLRWSNMATMPTLPETQAGIKEEIRRQEFLLNCLHRDLQGGIKDLSKEERLWEVQRILTALKRKLREAKRQECETKIAQEIASLSKEDVSKEEMNENEEVINILLAQENEILTEQEELLAMEQFLRRQIASEKEEIERLRAEIAEIQSRQQHGRSETEEYSSESESESEDEEELQIILEDLQRQNEELEIKNNHLNQAIHEEREAIIELRVQLRLLQMQRAKAEQQAQEDEEPEWRGGAVQPPRDGVLEPKAAREQPKAGKEPAKPSPSRDRKETSI
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (91); Rat (91)
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (97.79 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
-
Applications
Western Blot (Cell lysate)
RALBP1 monoclonal antibody (M02), clone 2A1 Western Blot analysis of RALBP1 expression in HeLa ( Cat # L013V1 ).Western Blot (Cell lysate)
RALBP1 monoclonal antibody (M02), clone 2A1. Western Blot analysis of RALBP1 expression in C32 ( Cat # L002V1 ).Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of RALBP1 transfected lysate using anti-RALBP1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RALBP1 MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged RALBP1 is approximately 0.03ng/ml as a capture antibody.ELISA
-
Gene Info — RALBP1
Entrez GeneID
10928GeneBank Accession#
BC013126Protein Accession#
AAH13126.1Gene Name
RALBP1
Gene Alias
RIP1, RLIP1, RLIP76
Gene Description
ralA binding protein 1
Omim ID
605801Gene Ontology
HyperlinkOther Designations
OTTHUMP00000162351|ral-interacting protein 1|ralA-binding protein 1
-
Interactomes
-
Pathways
-
Diseases
-
Publication Reference
-
Proteome Profiling Uncovers an Autoimmune Response Signature That Reflects Ovarian Cancer Pathogenesis.
Kobayashi M, Katayama H, Irajizad E, Vykoukal JV, Fahrmann JF, Kundnani DL, Yu CY, Cai Y, Hsiao FC, Yang WL, Lu Z, Celestino J, Long JP, Do KA, Lu KH, Ladd JJ, Urban N, Bast RC Jr, Hanash SM.
Cancers 2020 Feb; 12(2):E485.
Application:IP, WB-Tr, Human, DOV13, OVCAR8 cells.
-
Dual effects of Ral-activated pathways on p27 localization and TGF-β signaling.
Tazat K, Harsat M, Goldshmid-Shagal A, Ehrlich M, Henis YI.
Molecular Biology of the Cell 2013 Jun; 24(11):1812.
Application:WB-Ce, Human, Mv1Lu cells.
-
The RalB small GTPase mediates formation of invadopodia through a GTPase-activating protein-independent function of the RalBP1/RLIP76 effector.
Neel NF, Rossman KL, Martin TD, Hayes TK, Yeh JJ, Der CJ.
Molecular and Cellular Biology 2012 Apr; 32(8):1374.
Application:WB-Tr, Human, CFPAC-1 cells.
-
Dynamics of the subcellular localization of RalBP1/RLIP through the cell cycle: the role of targeting signals and of protein-protein interactions.
Fillatre J, Delacour D, Van Hove L, Bagarre T, Houssin N, Soulika M, Veitia RA, Moreau J.
FASEB Journal 2012 May; 26(5):2164.
Application:IF, WB-Tr, Human, Xenopus, X. laevis embryos, HeLa cells.
-
Ras-Related Small GTPases RalA and RalB Regulate Cellular Survival After Ionizing Radiation.
Kidd AR 3rd, Snider JL, Martin TD, Graboski SF, Der CJ, Cox AD.
International Journal of Radiation Oncology, Biology, Physics 2010 Sep; 78(1):205.
Application:WB, Human, MIA PaCa-2 cells.
-
Mass spectroscopic phosphoprotein mapping of Ral binding protein 1 (RalBP1/Rip1/RLIP76).
Herlevsen MC, Theodorescu D.
Biochemical and Biophysical Research Communications 2007 Aug; 362(1):56.
Application:IP-WB, Human, HEK 293T cells.
-
Expression of Ral GTPases, Their Effectors, and Activators in Human Bladder Cancer.
Smith SC, Oxford G, Baras AS, Owens C, Havaleshko D, Brautigan DL, Safo MK, Theodorescu D.
Clinical Cancer Research 2007 Jul; 13(13):3803.
Application:WB-Ti, Human, Adjacent normal bladder mucosa, muscle invasive bladder tumor.
-
Proteome Profiling Uncovers an Autoimmune Response Signature That Reflects Ovarian Cancer Pathogenesis.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com